Located in the Northern Tuli Game Reserve of Botswana which is situated between the Tuli Safari Area, a national park in Zimbabwe and the Mapungubwe National Park, a World Heritage Site in South Africa. Mashatu shares unfenced borders with both the South African and Zimbabwean national parks in the south and north respectively. This vast area forms part of a cross boundary wildlife conservation area protecting the substantial biodiversity of fauna and flora in this region.